Welcome to website for the Genomics Aotearoa ONT MinION workshop, which ran at the University of Otago, Dunedin, from 17th–19th April, 2018.

Schedule

Click here

Presentations

• Simone Cree:
  • Long read nanopore sequencing in the laboratory
• Chris Winefield:
  • Oxford Nanopore Sequencing in recalcitrant plant species
• Benjamin Schwessinger:
  • Insights into DNA extraction and library preparation for Nanopore sequencing on the MinION
• Louise Judd:
  • TBA
• Kirston Barton:
  • HMW DNA on Nanopore
  • Lab session: QCing your DNA before a library prep
• Alexis Lucattini:
  • Alignments, Quirks, and Assembly

Tutorials

1. Handling MinION data from your laptop
2. Setting up a virtual environment
3. Handling fast5 data on a server

Information for Participants

Click here

Sequencing results

• Here are some stats for each run, produced by Rob Lanfear’s MinIONQC.R script.

• There’s a .tar.gz file here with all the output from the script. The meaning of each plot is explained on the github repo.

• Here’s the weighted read length histogram for each sample.

• You can also find the sample QC and library yield results at the following links, to compare to the sequencing results:
  • Sample QC and library prep results PDF
  • Sample and sequencing spreadsheet

Links

• Nanopore group on protocols.io
  • Insect HMW DNA protocol used to extract samples 1–10
• Olin’s ultra cheap reasonably priced magnetic rack

Organisers

• Tom Harrop
  • Welcome slides
• Angela Hendry
• Andy Nilsen